Pilot scale production and characterization of pectinase enzyme fromAspergillus niger
نویسندگان
چکیده
منابع مشابه
Production kinetics and characterization of pectinase enzyme from Aspergillus niger
Food grade pectinases from Aspergillus niger are predominantly used as processing aids in industries. In the present investigation, efforts towards standardization for pilot scale production of pectinase enzyme from A. niger were made. The crude enzyme was studied for its kinetics to optimize substrate concentration and processing parameters on the basis of polygalacturonic activity. Km (Michae...
متن کاملISOLATION, PRODUCTION AND CHARACTERIZATION OF EXTRACELLULAR PECTINASE FROM Aspergillus niger K3
A pectinase producing fungus was isolated from compost soil. Morphological characteristics, microscopic observations and molecular characterization based on internal transcribed spacer (ITS) sequences led to its identification as Aspergillus niger (GenBank Accession Number: JX556221) and it was designated as Aspergillus niger strain K3. In the present study, the culture condition of the media w...
متن کاملAlkaline thermostable pectinase enzyme from Aspergillus niger strain MCAS2 isolated from Manaslu Conservation Area, Gorkha, Nepal
Pectinase enzymes are one of the commercially important enzymes having great potential in various industries especially in food industry. Pectinases accounts for 25 % of global food enzymes produced and their market is increasing day by day. Therefore, the exploration of microorganism with novel characteristics has always been the focus of the research. Microorganism dwelling in unique habitat ...
متن کاملIsolation, Identification and Enzyme Production Profile of A. Niger
Black Aspergilli (Aspergillus section Nigri) have agricultural and industrial importance. Several species of this group are recognized human pathogens and some are mycotoxin producers. Despite of their wide use in teaching and research, taxonomy of this group remains unclear due to very close morphological features. Present study is aimed to distinguish Aspergillus niger strains that were isola...
متن کاملAffinity precipitation of Aspergillus niger pectinase by microwave-treated alginate.
Affinity precipitation is a simple, single plate separation process in which the complex of a smart macroaffinity ligand with the target protein (from a crude broth) can be selectively precipitated by application of a suitable stimulus. Alginate is a copolymer of guluronic acid and mannuronic acid residues and precipitates with Ca(2+) ions. It was found to bind to pectinase present in a commerc...
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ژورنال
عنوان ژورنال: INTERNATIONAL JOURNAL OF PROCESSING AND POST HARVEST TECHNOLOGY
سال: 2015
ISSN: 0976-5638,2231-6426
DOI: 10.15740/has/ijppht/6.1/14-18